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Natugrain®
Definition of activities for
-glucanase and endo-xylanase
One
-glucanase-unit (BGU) is defined as the activity required to liberate 0.278 mmol reducing sugars (measured as glucose equivalents) per minute, from a 0.5%
-glucan solution at pH 3.5 and 40°C.
One endo-xylanase-unit (EXU) is defined as the enzyme activity required to liberate 1 mmol reducing sugars (measured as xylose equivalents) per minute from a 1 % xylan solution at pH 3,5 and 40°C.
Composition of Natugrain®
Natugrain® is an enzyme preparation originating from fungal fermentation (Trichoderma longibrachiatum). It contains p-glucanase and endo-xylanase as the main enzyme activities, which can cleave polysaccharides such as
-glucan from barley or pentosans from wheat. It further contains other hemicellulase (e.g. pectinase), cellulase-and proteinase activities. Therefore Natugrain® has wide applicability.
The granulate contains standardized activities of
-glucanase and endo-xylanase of at least 6,000 BGU/g and at least 8,250 EXU/g, respectively.
Application
Natugrain® was developed for use in feeds rich in cereals (wheat, triticale, rye, barley) for broilers, turkeys and laying hens. It counteracts the antinutritive properties of
-glucans and pentosans (arabinoxylans). These polysaccharides can result in increased viscosity of the chyme in the small intestine, and hence in lower utilization of nutrients. This problem can be solved by the addition of Natugrain®.
Non-starch polysaccharides (NSP) as antinutritive factors in poultry nutrition
The nutritive value of poultry feeds rich in cereals diminishes with increasing proportion of NSP. The most common kinds of NSP are pentosans and
-glucans. Pentosans occur primarily in cereals like wheat, rye and triticale, while barley and to a lesser extent oats contain relatively high concentrations of
-glucans (see table).
Non-starch polysaccharides and metabolizable energy content of selected cereals
| Cereal | -Glucans (g/kg) | Pentosans (g/kg) | Apparent metabolizable energy (AME) (MJ/kg DM) |
| Rice (dehulled) | 0.4 | 0 | 17.4 |
| Sorghum | 1.0 | 28.4 | 15.8 |
| Corn | 1.2 | 42.6 | 15.8 |
| Wheat | 5.0 | 60.5 | 14.3 |
| Triticale | 6.5 | 69.7 | 13.8 |
| Rye | 11.5 | 89.0 | 11.3 |
| Barley | 33.2 | 75.5 | 11.3 |
(modified from Choct and Annison, 1990)
Recent results from poultry experiments show that most wheat varieties and cultivars with low content of metabolizable energy (AME) also contain relatively high amounts of pentosans.
Because of the well-known gel-forming properties of water-soluble
-glucans und pentosans, diets rich in cereals are likely to lead to a marked increase in viscosity of the digesta. This is associated with reduced digestibility of nutrients, particularly of fat, and consequently poor performance and sticky droppings. These problems can be solved by the addition of Natugrain®. During passage of the digesta through the digestive tract, the enzyme results in partial hydrolysis of
-glucans and pentosans yielding smaller, less viscous polymers. This markedly lowers intestinal viscosity and consequently lessens the negative effects exerted by these polysaccharides in poultry diets.
Benefits of the use of Natugrain®
Addition of Natugrain® to high-cereal poultry diets has the following effects:
- Reduction of intestinal viscosity
- Improved absorption of nutrients
- Reduction in water intake (lower water-to-feed ratio)
- Less sticky droppings
Therefore, enzyme addition to poultry rations has the following benefits:
- Improved energy utilization from cereals and total feed
- Improved feed conversion
- Higher live weight gain
- Less variation in slaughter weight
- Improved litter quality
- Option to include freshly harvested grain in the feed
- More constants feed quality (lower variation in nutritive value between and within cereals)
- Higher flexibility in linear programming of feeds
Recommended dosage
There are sufficient data available for poultry to make reliable recommendations both from a physiological and an economic point of view. The rates of application for Natugrain® vary depending on the type of cereal and the rate of inclusion in the feed (see table). Thereby it has to be taken into account that the maximum recommended dose levels are 100 ppm Natugrain®, respectively - no matter if one or more cereal cultivars in the diet are above the minimum portion mentioned in the table below.
Recommended dosages for Natugrain® in broiler feeds rich in cereals
| Cereal | Cereal portion in diet (%) | Enzyme addition (ppm) Natugrain® |
| Wheat | 25-35 | 75 |
| Wheat | >35 | 100 |
| Triticale | >20 | 100 |
| Rye | >5 | 100 |
| Barley | >15 | 100 |
Stability of Natugrain® in premixes
The product has been designed to be compatible with nearly all feed components. However, premixes occasionally contain ingredients such as certain forms of choline chloride and trace element compounds rich in water of crystallization, which may affect enzyme activity. Further potential factors of influence originating from complex vitamin and trace element premixes are presently unknown. As a matter of caution, storage time in excess of 3 months should be avoided. Dry and cool storage is recommended.
Palletizing stability of Natugrain®
Based on performance experiments with broiler chickens, it is well established that Natugrain® has adequate pelletizing stability at pellet temperatures up to 85°C.
Packaging/storage
See page 135
Note
Inhaling of dust or aerosols of the product should be avoided, since in isolated cases allergic reactions in particularly sensitized people may occur. The usage of gloves and dust masks is recommended. Furthermore the formation of dust or aerosols when handling Natugrain® should be avoided as far as possible.
When the product has been spilled or come into contact with skin or eyes, rinse affected area throughly with water.
The instructions of the safety data sheets should be observed.
Natuphos in animal nutrition
Benefits of Natuphos
- Natuphos increases the digestibility of phosphorus
- Natuphos increases the digestibility of the complexed cations (Ca, Zn, Mg, Cu....)and reduces their excretion.
- Natuphos improves protein/amino acid digestibility
- Natuphos increases energie content
- Natuphos lowers feed cost
Natuphos decreases excreation of nutrients
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